Reduced NLRP3 Inflammasome Activity in PDE3B-KO mice is associated with increased insulin sensitivity

Authors

• F Ahmad
• Y Chung
• Y Tang
• S Hockman
• S Liu
• Y Khan
• K Huo
• E Billings
• M Amar
• A Remaley
• V Manganiello

Abstract

Activation of inflammation in white adipose tissue (WAT), includes infiltration/expansion of WAT macrophages, contributes pathogenesis of obesity, insulin resistance, and metabolic syndrome. Inflammasome activation leads to maturation of caspase-1 and processing of IL1β, contributing to many metabolic disorders and directing adipocytes to a more insulin-resistant phenotype. Ablation of PDE3B in WAT prevents inflammasome activation by reducing the expression of NLRP3, caspase-1, ASC, AIM2, TNFα, IL1β and proinflammatory genes. Following IP injection of lipopolysaccharide (LPS), serum levels of IL1β and TNFα were reduced in PDE3B-/- mice compared to WT. Activation of signaling cascades, which mediate inflammasome responses, were modulated in PDE3B-/- mice WAT, including smad, NFAT, NFkB, and MAP kinases. Moreover, expression of chemokine CCL2, MCP-1 and its receptor CCR2, which play an important role in macrophage chemotaxis, were reduced in WAT of PDE3B-/- mice. PDE3B-/- mice exhibit significantly increased insulin sensitivity. In addition, atherosclerotic plaque formation was significantly reduced in the aorta of apoE-/-/PDE3B-/- and LDL-R-/-/PDE3B-/- mice compared to apoE-/- and LDL-R-/- mice, respectively. Collectively, these data establish a role for PDE3B in modulating inflammatory response, which may contribute to a reduced inflammatory state in adipose tissue.

Scientific Focus Area: Molecular Biology and Biochemistry

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