Genome-wide approaches elucidate the role of Ikaros in macrophage LPS responses

Authors

• KS Oh
• RA Gottschalk
• SJ Baek
• GL Hager
• MH Sung
• ID Fraser

Abstract

The transcription factor Ikaros is an essential regulator of lymphopoiesis. However, due to decreased expression during differentiation of the myeloid lineage, Ikaros function in macrophages has not been considered important. Previously, we identified a novel role for Ikaros in regulating the inflammatory gene program in LPS activated macrophages. To gain insight into functions and mechanisms of Ikaros action, we performed genome-wide assays using next generation sequencing technology including Chromatin Immunoprecipitation (ChIP)-seq, RNA-seq and DNase-seq analysis of LPS stimulated bone marrow derived macrophages from WT and Ikaros KO mice. Initial analysis of these data showed that important subsets of LPS-induced genes are attenuated in Ikaros KO macrophages and also highlighted LPS-induced dynamic changes in chromatin accessibility (DHS-seq) that are influenced by Ikaros deficiency.

Scientific Focus Area: Systems Biology

This page was last updated on Friday, March 26, 2021